Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed.

@article{Mita2001MolecularCA,
  title={Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed.},
  author={Giovanni Mita and Antonia Gallo and Valentina Greco and C Zasiura and Rod Casey and Giuseppe Zacheo and Angelo Santino},
  journal={European journal of biochemistry},
  year={2001},
  volume={268 5},
  pages={1500-7}
}
We have characterized an almond (Prunus dulcis) lipoxygenase (LOX) that is expressed early in seed development. The presence of an active lipoxygenase was confirmed by western blot analysis and by measuring the enzymatic activity in microsomal and soluble protein samples purified from almond seeds at this stage of development. The almond lipoxygenase, which had a pH optimum around 6, was identified as a 9-LOX on the basis of the isomers of linoleic acid hydroperoxides produced in the enzymatic… CONTINUE READING

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