Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent alpha-glycerol-3-phosphate dehydrogenase.

@article{Menaya1995MolecularCS,
  title={Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent alpha-glycerol-3-phosphate dehydrogenase.},
  author={J. M. Garc{\'i}a Menaya and Consuelo Gonz{\'a}lez-Manch{\'o}n and Roberto Parrilla and Matilde S. Ayuso},
  journal={Biochimica et biophysica acta},
  year={1995},
  volume={1262 1},
  pages={91-4}
}
This work reports the primary nucleotide structure and in vitro translation of a cDNA, expressed by a gene mapping on chromosome 12, that encodes a human hepatic alpha-glycerol-3-phosphate dehydrogenase (L-glycerol-3-phosphate:NAD oxidoreductase, E.C. 1.1.1.8). The 1413 bp cDNA comprises an ORF of 1050 bp that encodes a 349 amino acid protein of 37.5 kDa. Northern blot analysis of poly(A)+ mRNA from human liver showed three transcripts, while from human placenta only two transcripts were… CONTINUE READING