Molecular cloning, sequencing and expression in Escherichia coli of the capsid protein gene from rabbit haemorrhagic disease virus (Spanish isolate AST/89).

@article{Boga1994MolecularCS,
  title={Molecular cloning, sequencing and expression in Escherichia coli of the capsid protein gene from rabbit haemorrhagic disease virus (Spanish isolate AST/89).},
  author={Jos{\'e} Antonio Boga and R C B Casais and M Soledad Mar{\'i}n and Jos{\'e} Manuel Mart{\'i}n-Alonso and R. S. Carmenes and Miguel Prieto and Francisco Vargas Parra},
  journal={The Journal of general virology},
  year={1994},
  volume={75 ( Pt 9)},
  pages={2409-13}
}
We describe the cloning, nucleotide sequencing and expression in Escherichia coli of the major capsid component (VP60) from the Spanish field isolate AST/89 of rabbit haemorrhagic disease virus (RHDV). The sequence of the 3'-terminal 2483 nucleotides of the genome was found to be 95.4% identical to the German RHDV strain, showing ten changes in the deduced… CONTINUE READING