Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis.

@article{Deng2013MolecularCF,
  title={Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis.},
  author={Wei-wei Deng and Ming Zhang and Jian-Qiang Wu and Zheng-Zhong Jiang and Lei Tang and Y. Li and Chaoling Wei and C Jiang and Xiao-chun Wan},
  journal={Journal of plant physiology},
  year={2013},
  volume={170 3},
  pages={272-82}
}
Cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) is considered to be a key enzyme in lignin biosynthesis, but little was known about CADs in tea plants (Camellia sinensis). A full-length cDNA sequence (CsCAD2) was isolated by suppressive subtractive hybridization (SSH) in Ectropis oblique feeding-induced tea plants, and another two full-length cDNA sequences (CsCAD1 and CsCAD3) were obtained from a transcriptome obtained by deep sequencing. However, they showed only 20-54% identities… CONTINUE READING