Molecular cloning, expression and tissue distribution of hamster diacetyl reductase. Identity with L-xylulose reductase.

@article{Ishikura2001MolecularCE,
  title={Molecular cloning, expression and tissue distribution of hamster diacetyl reductase. Identity with L-xylulose reductase.},
  author={Shuhei Ishikura and Tomoya Isaji and Noriyuki Usami and Kanefumi Kitahara and Junichi Nakagawa and Akira Hara},
  journal={Chemico-biological interactions},
  year={2001},
  volume={130-132 1-3},
  pages={879-89}
}
Using rapid amplification of cDNA ends PCR, a cDNA species for diacetyl reductase (EC 1.1.1.5) was isolated from hamster liver. The encoded protein consisted of 244 amino acids, and showed high sequence identity to mouse lung carbonyl reductase and hamster sperm P26h protein, which belong to the short-chain dehydrogenase/reductase family. The enzyme efficiently reduced L-xylulose as well as diacetyl, and slowly oxidized xylitol. The K(m) values for L-xylulose and xylitol were similar to those… CONTINUE READING

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