Molecular cloning, expression and regional distribution of rat ciliary neurotrophic factor

  title={Molecular cloning, expression and regional distribution of rat ciliary neurotrophic factor},
  author={Kurt A. St{\"o}ckli and Friedrich Lottspeich and Michael Sendtner and Piotr Masiakowski and Patrick Carroll and Rudolf Götz and D. B. Lindholm and Hans Thoenen},
CILIARY neurotrophic factor (CNTF) was originally characterized as a survival factor for chick ciliary neurons in vitro1. More recently, it was shown to promote the survival of a variety of other neuronal cell types2,3 and to affect the differentiation of E7 chick sympathetic neurons by inhibiting their proliferation and by inducing the expression of vasoactive intestinal peptide immunoreac-tivity (VIP-IR) 4. In cultures of dissociated sympathetic neurons from newborn rats, CNTF induces… 
Constitutive expression of ciliary neurotrophic factor in mouse hypothalamus
In normal adult mice the ependyma of the third ventricle is both a source of and a target for CNTF, which may play hitherto unknown roles in hypothalamic function in physiological conditions.
Effect of ciliary neurotrophic factor (CNTF) on motoneuron survival
Summary We have demonstrated that the extensive degeneration of motoneurons in the rat facial nucleus after transection of the facial nerve in newborn rats can be prevented by local ciliary
Regional distribution, developmental changes, and cellular localization of CNTF-mRNA and protein in the rat brain
It is demonstrated that the time course of the expression ofCNTF-mRNA and protein in the rat optic nerve is compatible with such a glial differentiation function of CNTF in vivo, and it is shown that the type 2 astrocyte-inducing activity previously demonstrated in optic nerve extract can be precipitated by an antiserum against CNTF.
Synthesis and localization of ciliary neurotrophic factor in the sciatic nerve of the adult rat after lesion and during regeneration
It is demonstrated that the CNTF mRNA and protein levels and the manner in which they are regulated are compatible with such a function in lesioned peripheral neurons in the adult peripheral nervous system.
Cloning and expression of human ciliary neurotrophic factor.
The cloning of the entire genomic sequence encoding human CNTF and its primary structure is reported, providing a basis for understanding the role of CNTF in nervous system physiology and pathology.
Ciliary neurotrophic factor enhances neuronal survival in embryonic rat hippocampal cultures
Investigation of neurotrophic effects of CNTF toward three populations of rat hippocampal neurons shows an increase in the neurofilament content of hippocampal cultures prepared from embryonic day 18 (E18) rat brain, and CNTF increased high-affinity GABA uptake and glutamic acid decarboxylase activity.


Ciliary neurotrophic factor induces cholinergic differentiation of rat sympathetic neurons in culture
The effects of CNTF on ChAT and TH are not due to selective survival of cells that acquire cholinergic traits in vitro, but are rather due to the induction of cholinergy differentiation of noradrenergic sympathetic neurons.
Molecular cloning and expression of brain-derived neurotrophic factor
The full primary structure of brain-derived neurotrophic factor is reported and it is established that these two neurotrophic factors are related both functionally and structurally.
Astroglial and fibroblast growth factors have neurotrophic functions for cultured peripheral and central nervous system neurons.
The potency of a class of mitogenic growth factors as neurotrophic agents for embryonic ciliary ganglion and spinal cord neurons is demonstrated, adding to the emerging evidence that mitogenic and neuronal growth factors are not strictly separate entities.
Ciliary neurotrophic factor induces type-2 astrocyte differentiation in culture
It is shown that the type-2-astrocyte-inducing protein is similar or identical to ciliary neutrotrophic factor (CNTF)8,9, which promotes the survival of some types of peripheral neurons in vitro8, including ciliary ganglion neurons8,10, which suggests that CNTF, in addition to its effect on neurons, may be responsible for triggering type-1 astrocytes differentiation in the developing CNS.
Cholinergic neuronotrophic factors: intraocular distribution of trophic activity for ciliary neurons.
An assay system based on microwell cultures of ciliary ganglionic neurons was used to demonstrate that a very rich source of trophic factor for them is the intraocular target tissues they innervate, and suggests that this soluble factor may be involved in the normal development of the ciliary Ganglion.
Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.
An oligonucleotide probe was designed from the nucleotide sequence of the amino-terminal exon of bovine acidic FGF, taking into account the 55 percent amino acid sequence homology between the two factors.
Purification of the Chick Eye Ciliary Neuronotrophic Factor
Purified CNTF represents the first purified neuronotrophic factor which addresses parasympathetic cholinergic neurons and supports the 24‐h survival of cultured neurons from certain chick and rodent sensory and sympathetic ganglia.