Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2.

@article{Bohm1996MolecularCE,
  title={Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2.},
  author={S K Bohm and Wei Kong and Dieter Bromme and S. P. Smeekens and Donald C. Anderson and Andrew Connolly and Mark Kahn and Nicholas A Nelken and Shaun R Coughlin and Donald G. Payan and Nigel W Bunnett},
  journal={The Biochemical journal},
  year={1996},
  volume={314 ( Pt 3)},
  pages={1009-16}
}
We used PCR to amplify proteinase activated receptor-2 (PAR-2) from human kidney cDNA. The open reading frame comprised 1191 bp and encoded a protein of 397 residues with 83% identity with mouse PAR-2. In KNRK cells (a line of kirsten murine sarcoma virus-transformed rat kidney epithelial cells) transfected with this cDNA, trypsin and activating peptide (AP) corresponding to the tethered ligand exposed by trypsin cleavage (SLIGKV-NH2) induced a prompt increase in cytosolic calcium ion… CONTINUE READING
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