Molecular cloning, expression and characterization of the porcine β defensin 2 in E. coli.

@article{Li2013MolecularCE,
  title={Molecular cloning, expression and characterization of the porcine β defensin 2 in E. coli.},
  author={Chun-li Li and Yan-cong Zhao and Xiao-yan Song and Xian-xian Huang and Wei-dong Zhao},
  journal={Protein and peptide letters},
  year={2013},
  volume={20 6},
  pages={715-23}
}
Porcine β defensin 2(pBD2)is a cationic 37-amino acid antimicrobial peptide with disulfide bonds. Synthetic pBD2 had broad antimicrobial activity against pathogenic bacteria, and thus pBD2 could be a good candidate as a bactericidal agent for pigs. This study reported the successful recombinant expression of pBD2 in Escherichia coli and analysis of its antimicrobial activity, its hemolytic activity, salt-tolerance and thermal stability as well. The pBD2 gene, obtained by RT-PCR using the tongue… CONTINUE READING