Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis.

@article{Asawatreratanakul2003MolecularCE,
  title={Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis.},
  author={Kasem Asawatreratanakul and Yuan-wei Zhang and Dhirayos Wititsuwannakul and Rapepun Wititsuwannakul and Seiji Takahashi and Atiya Rattanapittayaporn and Tanetoshi Koyama},
  journal={European journal of biochemistry},
  year={2003},
  volume={270 23},
  pages={4671-80}
}
Natural rubber from Hevea brasiliensis is a high molecular mass polymer of isoprene units with cis-configuration. The enzyme responsible for the cis-1,4-polymerization of isoprene units has been idengified as a particle-bound rubber transferase, but no gene encoding this enzyme has been cloned from rubber-producing plants. By using sequence information from the conserved regions of cis-prenyl chain elongating enzymes that were cloned recently, we have isolated and characterized cDNAs from H… CONTINUE READING

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