Molecular cloning, expression, and purification of nuclear inclusion A protease from tobacco vein mottling virus.

@article{Hwang2000MolecularCE,
  title={Molecular cloning, expression, and purification of nuclear inclusion A protease from tobacco vein mottling virus.},
  author={Daye Hwang and Doo Hwan Kim and Byoung Heon Kang and Byeong Doo Song and Kwan Yong Choi},
  journal={Molecules and cells},
  year={2000},
  volume={10 2},
  pages={148-55}
}
The gene encoding the C-terminal protease domain of the nuclear inclusion protein a (NIa) of tobacco vein mottling virus (TVMV) was cloned from an isolated virus particle and expressed as a fusion protein with glutathione S-transferase in Escherichia coli XL1-blue. The 27-kDa protease was purified from the fusion protein by glutathione affinity chromatography and Mono S chromatography. The purified protease exhibited the specific proteolytic activity towards the nonapeptide substrates, Ac-Glu… CONTINUE READING

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