Molecular cloning, characterization of cDNA, and distribution of mRNA encoding the frog prohormone convertase PC1.

@article{Gangnon1999MolecularCC,
  title={Molecular cloning, characterization of cDNA, and distribution of mRNA encoding the frog prohormone convertase PC1.},
  author={Françoise Gangnon and J. M. Danger and Sylvie J{\'e}gou and Didier Vieau and Nabil G Seidah and Hubert Vaudry},
  journal={The Journal of comparative neurology},
  year={1999},
  volume={405 2},
  pages={
          160-72
        }
}
Prohormone convertases (PCs) are calcium-dependent serine endoproteases of the subtilisin/kexin family that play a key role in the posttranslational processing of precursors for biologically active peptides. In this study, we have characterized the cDNA encoding PC1 in the European green frog Rana ridibunda. A frog brain cDNA library was screened by using a heterologous probe at low stringency, and a 2.3-kb cDNA clone encoding PC1 was isolated. This cDNA encodes a 736-residue protein with a 26… CONTINUE READING