Molecular cloning, characterization, and sequencing of the hemolysin gene from Edwardsiella tarda

@article{Chen1996MolecularCC,
  title={Molecular cloning, characterization, and sequencing of the hemolysin gene from Edwardsiella tarda},
  author={J.-D. Chen and Shau-Yan Lai and S C Huang},
  journal={Archives of Microbiology},
  year={1996},
  volume={165},
  pages={9-17}
}
Hemolysis is a major symptom of diseased eels infected by Edwardsiella tarda. The hemolysin gene of E. tarda strain ET16 was cloned into plasmid pSK and expressed in Escherichia coli. The mol. mass of the functional β-hemolysin was estimated to be approximately 34 kDa by gel filtration and by SDS-PAGE followed by in situ hemolysin activity analysis. The cloned fragment containing the β-hemolysin locus from E. tarda strain ET16 expressed in E. coli was estimated to be 5.3 kb in length; the… CONTINUE READING

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Cloning and characterization of the haemolysin determinants from Aeromonas hydrophila

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