Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation.

  title={Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation.},
  author={M. Ruat and E. Traiffort and R. Leurs and J. Tardivel-Lacombe and J. D{\'i}az and J. Arrang and J. Schwartz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={90 18},
  • M. Ruat, E. Traiffort, +4 authors J. Schwartz
  • Published 1993
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
By using a strategy based on nucleotide sequence homology, we have cloned a cDNA encoding a functional serotonin (5-HT) receptor. The deduced amino acid sequence of the 5-HT7 receptor displays limited homology with that of other 5-HT receptors. In addition to the seven stretches of hydrophobic amino acids that characterize the superfamily of receptors interacting with guanine nucleotide-binding proteins, the 448-aa sequence of the 5-HT7 receptor contains a hydrophobic domain located at its N… Expand
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