Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation.

  title={Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation.},
  author={Martial Ruat and Elisabeth Traiffort and Rob Leurs and Jo{\"e}l Tardivel-Lacombe and J. D{\'i}az and J-M. Arrang and J-C. Schwartz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={90 18},
  • M. RuatE. Traiffort J. Schwartz
  • Published 15 September 1993
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
By using a strategy based on nucleotide sequence homology, we have cloned a cDNA encoding a functional serotonin (5-HT) receptor. The deduced amino acid sequence of the 5-HT7 receptor displays limited homology with that of other 5-HT receptors. In addition to the seven stretches of hydrophobic amino acids that characterize the superfamily of receptors interacting with guanine nucleotide-binding proteins, the 448-aa sequence of the 5-HT7 receptor contains a hydrophobic domain located at its N… 

Figures and Tables from this paper

Cloning and Functional Expression of an Aplysia 5-HT Receptor Negatively Coupled to Adenylate Cyclase

The cloning of a cDNA coding for an Aplysia G-protein-coupled 5-HT receptor (5-HTap1) is reported, whose deduced amino acid sequence resembles those of the 5- HT1 receptor subfamily.

A human serotonin-7 receptor pseudogene.

Pharmacological characterization of a serotonin receptor (5-HT7) stimulating cAMP production in human corneal epithelial cells.

Collective data support the presence of a pharmacologically defined, adenylyl cyclase-coupled 5-HT7 receptor in the CEPI-17-CL4 cells that may have relevance to physiological and/or pathologic functions of 5- HT7 receptors in the human cornea.

Molecular cloning and tissue distribution of mRNA encoding procine 5-HT7 receptor and its comparison with the structure of other species

The established cDNA sequence and tissue distribution of porcine 5-HT7 receptor will be helpful in exploring the role of this receptor in pathophysiological processes and to predict as a potential therapeutic target for antimigraine drug development.

Molecular Biology and Genomic Organization of G Protein-Coupled Serotonin Receptors

This chapter summarizes naturally occurring variation in serotonin receptor sequences, which vary by species and by class and subclass and are further modified from their canonical sequences by RNA editing, alternative splicing, and the existence of single-nucleotide polymorphisms.

The cloned human 5-HT7 receptor splice variants: a comparative characterization of their pharmacology, function and distribution

It is indicated that the three human splice variants are pharmacologically indistinguishable and that modifications of the carboxyl tail do not influence coupling to adenylyl cyclase.


Serotonin receptor subtypes : pharmacological significance and clinical implications

The 5-HT receptors modulate glutamate release in cerebellum and cholecystokinin release in nucleus accumbens and possible relevance to cerebellar ataxias and to anxiety, M. Raiteri et al serotonin and eating disorders - pharmacological relationships.