Molecular cloning, characterization, and expression of a novel human neutral sphingomyelinase.

@article{Chatterjee1999MolecularCC,
  title={Molecular cloning, characterization, and expression of a novel human neutral sphingomyelinase.},
  author={Subroto Chatterjee and Hong Peng Han and Shadon Rollins and Tavia Cleveland},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 52},
  pages={37407-12}
}
Neutral sphingomyelinase (N-SMase) has emerged as an important cell membrane-associated enzyme that participates in several signal transduction and cell regulatory phenomena. Using expression cloning, we have identified a 3.7-kilobase pair cDNA transcript for N-SMase whose open reading frame predicts a 397-amino acid polypeptide. Transfection of COS-7 cells with cDNA for N-SMase resulted in a marked increase in N-SMase activity. Recombinant N-SMase (r-N-SMase) had the following physical… CONTINUE READING