Molecular characterization of resistance-nodulation-division transporters from solvent- and drug-resistant bacteria in petroleum-contaminated soil.

@article{Meguro2005MolecularCO,
  title={Molecular characterization of resistance-nodulation-division transporters from solvent- and drug-resistant bacteria in petroleum-contaminated soil.},
  author={Norika Meguro and Yumiko Kodama and M. Elisabeth Hern{\'a}ndez Gallegos and Kazuya Watanabe},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 1},
  pages={580-6}
}
PCR assays for analyzing resistance-nodulation-division transporters from solvent- and drug-resistant bacteria in soil were developed. Sequence analysis of amplicons showed that the PCR successfully retrieved transporter gene fragments from soil. Most of the genes retrieved from petroleum-contaminated soils formed a cluster (cluster PCS) that was distantly related to known transporter genes. Competitive PCR showed that the abundance of PCS genes is increased in petroleum-contaminated soil.