Molecular characterization of tobacco squalene synthase and regulation in response to fungal elicitor.

@article{Devarenne1998MolecularCO,
  title={Molecular characterization of tobacco squalene synthase and regulation in response to fungal elicitor.},
  author={Timothy P Devarenne and D. H. Shin and Kyoungwhan Back and Shaohui Yin and Joseph Chappell},
  journal={Archives of biochemistry and biophysics},
  year={1998},
  volume={349 2},
  pages={
          205-15
        }
}
The enzyme squalene synthase (SS) represents the first commitment of carbon from the general isoprenoid pathway toward sterol biosynthesis and is a potential point for regulation of sterol biosynthesis. The isolation and characterization of tobacco (Nicotiana tabacum) squalene synthase (TSS) cDNA and genomic DNA clones, as well as determination of the steady state level of TSS mRNA in response to elicitor treatment, were investigated. cDNA clones for TSS were isolated from poly (A)+ RNA using a… 
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Regulation of Squalene Synthase, a Key Enzyme of Sterol Biosynthesis, in Tobacco1
TLDR
SS enzyme activity, polypeptide level, and gene expression were all localized predominately to the shoot apical meristem, with much lower levels observed in leaves and roots, which suggest that sterol biosynthesis is localized to the apicalMeristems and that apical Meristems may be a source of sterols for other plant tissues.
Molecular cloning and characterization of the yew gene encoding squalene synthase from Taxus cuspidata.
TLDR
Bioinformatic analysis and northern blotting analysis revealed that the deduced TcSqS protein had high similarity with other plant squalene synthases and a predicted crystal structure similar to other class I isoprenoid biosynthetic enzymes.
Molecular cloning, bacterial expression and promoter analysis of squalene synthase from Withania somnifera (L.) Dunal.
Molecular characterization of squalene synthase from the green microalga Botryococcus braunii, race B.
TLDR
Results suggest either that there exists coordinate expression of separate synthase genes for squalene and botryococcene biosynthesis or that there might be unique physiological conditions controlling the SS vs botryOCoccene synthase activity of a single peptide species.
Cloning, Expression Analysis and Functional Characterization of Squalene Synthase (SQS) from Tripterygium wilfordii
TLDR
The results indicated that TwSQS was highly expressed in roots, followed by the stems and leaves, and was significantly up-regulated upon MeJA treatment, which meant that TwsQS did have catalytic activity.
Molecular cloning and differential expression analysis of a squalene synthase gene from Dioscoreazingiberensis, an important pharmaceutical plant
TLDR
Quantitative real-time PCR analysis revealed that DzSQS was expressed from highest to lowest order in mature leaves, newly-formed rhizomes, young leaves, young stems, and two-year-old rhizome of D. zingiberensis.
Cloning, overexpression and characterization of Leishmania donovani squalene synthase.
TLDR
This is the first report on the purification and characterization of full-length recombinant SSN from L. donovani, and studies on recombinant LdSSN will help in evaluating this enzyme as a potential drug target for visceral leishmaniasis.
Cloning and Functional Characterization of a Squalene Synthase from Paris polyphylla var. yunnanensis
TLDR
Gas chromatography‐mass spectrometry analysis showed that the purified recombinant PpSQS1 protein could produce squalene using FDP as a substrate in the in vitro enzymatic assay, suggesting that Pp SQS 1 is likely involved in the biosynthesis of steroidal saponins in the plant.
Cloning, expression and characterization of squalene synthase from Inonotus obliquus
TLDR
The result of this study could serve as an important step toward the manipulation of triterpenoids biosynthesis in I. obliquus at the level of squalene through engineering better SQS for reintroduction into the mushroom.
Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (Amaranthus cruentus L.)
TLDR
The findings showed that the amaranth SQS is a late-expressed gene that is rapidly expressed at the mid-late stage of seed development, and it was observed that the SQS mRNA levels in stems and roots increased rapidly during the fourto six-leaf stage of development.
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