Molecular characterization of the t(3;9) associated with immortalization in the MCF10A cell line.

@article{Cowell2005MolecularCO,
  title={Molecular characterization of the t(3;9) associated with immortalization in the MCF10A cell line.},
  author={John K Cowell and Jeffrey R. Laduca and Michael R. Rossi and Tania Burkhardt and Norma J. Nowak and Sei-ichi Matsui},
  journal={Cancer genetics and cytogenetics},
  year={2005},
  volume={163 1},
  pages={23-9}
}
The t(3;9)(p14;p21) in the MCF10A human mammary gland epithelial cell line was the single cytogenetic event that accompanied the transition from primary culture to immortalized cell line, suggesting that it is related to the development of the immortalization phenotype. To study the molecular consequences of the breakpoints in this rearrangement, we used a combination of fluorescence in situ hybridization (FISH) and array comparative genomic hybridization (CGH). The 3p14 translocation… CONTINUE READING