Molecular characterization of the lincomycin‐production gene cluster of Streptomyces lincolnensis 78‐11

@article{Peschke1995MolecularCO,
  title={Molecular characterization of the lincomycin‐production gene cluster of Streptomyces lincolnensis 78‐11},
  author={Ursula Peschke and Heike Schmidt and Hua Zhong Zhang and W. Piepersberg},
  journal={Molecular Microbiology},
  year={1995},
  volume={16}
}
The lincomycin (LM)‐production gene cluster of the overproducing strain Streptomyces iincolnensis 78‐11 was cloned, analysed by hybridization, as well as by DNA sequencing, and compared with the respective genome segments of other lincomycin producers. The lmb/lmr gene cluster is composed of 27 open reading frames with putative biosynthetic or regulatory functions (lmb genes) and three resistance (lmr) genes, two of which, lmrA and lmrC, flank the cluster. A very similar overall organization of… Expand
Putative lmbI and lmbH genes form a single lmbIH ORF in Streptomyces lincolnensis type strain ATCC 25466
TLDR
The present study demonstrates the presence of a single larger open reading frame, called LmbIH, in the lincomycin low-production type strain Streptomyces lincolnensis ATCC 25466, instead of two smaller lmbI and lmbH genes, which is a protein of an unknown function and is homologous with the TldD protein family. Expand
Comparative transcriptomic analysis reveals the significant pleiotropic regulatory effects of LmbU on lincomycin biosynthesis
TLDR
LmbU is a significant pleiotropic transcriptional regulator in lincomycin biosynthesis by entirely activating the lmb cluster and regulating the non- lmb genes in Streptomyces lincolnensis, and shed new light on the transcriptional effects of LmbU-like family proteins on antibiotic biosynthesis in actinomycetes. Expand
The Novel Transcriptional Regulator LmbU Promotes Lincomycin Biosynthesis through Regulating Expression of Its Target Genes in Streptomyces lincolnensis
TLDR
This study identifies LmbU as a novel transcriptional regulator and provides new insights into regulation of lincomycin biosynthesis in S. lincolnensis and shows that this new regulatory element is relatively widespread across diverse actinomycetes species. Expand
A putative redox‐sensing regulator Rex regulates lincomycin biosynthesis in Streptomyces lincolnensis
TLDR
In this study, a redox-sensing regulator Rex from Streptomyces lincolnensis (Rexlin) was identified and characterized to affect cell growth and lincomycin biosynthesis. Expand
Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466
TLDR
A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced and analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC25466 strain. Expand
Amplification of the entire kanamycin biosynthetic gene cluster during empirical strain improvement of Streptomyces kanamyceticus.
  • K. Yanai, T. Murakami, M. Bibb
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2006
TLDR
Southern analysis and DNA sequencing revealed amplification of a large genomic segment including the entire Km biosynthetic gene cluster in the chromosome of strain 12-6, suggesting that DNA amplification occurred during strain improvement as a consequence of selection for increased Km resistance. Expand
The Actinomycin Biosynthetic Gene Cluster of Streptomyces chrysomallus: a Genetic Hall of Mirrors for Synthesis of a Molecule with Mirror Symmetry
TLDR
Phylogenetic analysis indicates that the pathway-specific tryptophan metabolism in Streptomyces most probably evolved divergently from the normal pathway of tryptophile catabolism to provide an extra or independent supply of building blocks for the synthesis of tryPTophan-derived secondary metabolites. Expand
The genes lmbB1 and lmbB2 of Streptomyces lincolnensis encode enzymes involved in the conversion of l-tyrosine to propylproline during the biosynthesis of the antibiotic lincomycin A
Abstract The genes lmbA,B1,B2 in the lincomycin A production gene cluster of Streptomyces lincolnensis were shown to form a common transcription unit with the promoter located directly upstream ofExpand
ZouA, a Putative Relaxase, Is Essential for DNA Amplification in Streptomyces kanamyceticus
TLDR
It is proposed that DNA amplification in S. kanamyceticus is initiated by relaxase-mediated recombination between oriT-related sequences, which might function in a manner similar to that of oriT. Expand
Enhanced production of validamycin A in Streptomyces hygroscopicus 5008 by engineering validamycin biosynthetic gene cluster
TLDR
It is demonstrated that the amplification of the val gene cluster was an efficient strategy to enhance VAL-A production by S. hygroscopicus 5008, and the information obtained would be helpful for engineering other interesting antibiotic biosynthesis by gene cluster amplification. Expand
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