Molecular characterization of the Erwinia chrysanthemi kdgK gene involved in pectin degradation

@article{HugouvieuxCottePattat1994MolecularCO,
  title={Molecular characterization of the Erwinia chrysanthemi kdgK gene involved in pectin degradation},
  author={N. Hugouvieux-Cotte-Pattat and W. Nasser and J. Robert-Baudouy},
  journal={Journal of Bacteriology},
  year={1994},
  volume={176},
  pages={2386 - 2392}
}
The pathways of pectin and galacturonate catabolism in Erwinia chrysanthemi converge to form a common intermediate, 2-keto-3-deoxygluconate (KDG), which is phosphorylated by KDG kinase encoded by the kdgK gene. We cloned the kdgK gene of E. chrysanthemi 3937 by complementing an Escherichia coli kdgK mutation, using an RP4-derivative plasmid. One of the kdgK R-prime plasmids harbored a DNA insert of about 80 kb and carried the uxuA and uxuB genes involved in glucuronate catabolism and the celY… Expand
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Molecular analysis of the Erwinia chrysanthemi region containing the kdgA and zwf genes
TLDR
The pathways of pectin and galacturonate catabolism in Erwinia chrysantheml converge to form a common intermediate, 2‐keto‐3‐deoxygluconate (KDGP) which is phosphorylated by the aldolase encoded by the kdgA gene, and shown to be highly conserved in E. coli, Zymomonas mobilis and Pseudomonas putida enzymes. Expand
Characterization of kdgR, a gene of Erwinia chrysanthemi that regulates pectin degradation
TLDR
The 25 bp oligonucleotide AAAAAAGAAACATTG‐TTTCATTTGT corresponding to this consensus was substituted to the lac operator, at the beginning of transcription of the lacZ gene, which functioned as an operator for binding of the KdgR protein in vivo. Expand
Purification and functional characterization of the KdgR protein, a major repressor of pectinolysis genes of Erwinia chrysanthemi
TLDR
Results suggest the existence of a specific interaction between KDG and KdgR protein, an actual inducer of pectinolysis, which releases the repressor from the operator complexes, whereas galacturonate, which is the precursor of the actual inducers, does not. Expand
Isolation of Erwinia chrysanthemi kduD mutants altered in pectin degradation
TLDR
Analysis of the kduD::Mu d(Ap lac) insertions indicated that kduS is either an isolated gene or the last gene of a polycistronic operon, and beta-galactosidase activity was shown to be sensitive to catabolite repression by glucose and to be inducible by polygalacturonate, galacturonates, and other intermediates of polygalactic catabolism. Expand
Nucleotide sequences of the Erwinia chrysanthemi ogl and pelE genes negatively regulated by the kdgR gene product.
TLDR
Analysis of the regulatory regions of ogl and pelE allowed us to identify possible CRP-binding sites for these two genes, and a sequence 3' to the coding region was found to be able to form a secondary structure which may function as an Rho-independent transcriptional termination signal. Expand
Isolation of Erwinia chrysanthemi mutants altered in pectinolytic enzyme production
TLDR
It is confirmed that 2‐keto‐3‐deoxy‐gluconate is a key intermediate for the induction of the pectin catabolic pathway. Expand
Analysis of an Erwinia chrysanthemi gene cluster involved in pectin degradation
TLDR
A comparison of the regulatory regions of all the genes controlled by kdgR allowed better definition of the KdgC‐binding‐site consensus, which could have a role in the pathogenicity of E. chrysanthemi. Expand
Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis.
TLDR
Despite the presence of potential KdgR binding sites in the regulatory regions of four genes involved in pectin catabolism, no DNA-repressor complex could be observed by in vitro experiments, suggesting the existence of different regulation mechanisms mediated by the KdGR protein for the two classes of operators. Expand
Isolation of kdgK-lac and kdgA-lac Gene Fusions in the Phytopathogenic Bacterium Erwinia chrysanthemi
TLDR
Synthesis of β-galactosidase in these strains was induced in the presence of galacturonate, glucuronato, polygalacturonates or some intermediates of the catabolism of these sugars in the culture medium; synthesis ofβ-galactsidase was not sensitive to glucose repression. Expand
Hexuronate catabolism in Erwinia chrysanthemi
TLDR
In the phytopathogenic enterobacterium Erwinia chrysanthemi, the catabolism of hexuronates is linked to the degradation of pectic polymers and KDG, the common end product of both pathways, is cleaved by the kdGK and kdgA gene products. Expand
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