Molecular characterization of synaptophysin, a major calcium‐binding protein of the synaptic vesicle membrane.

  title={Molecular characterization of synaptophysin, a major calcium‐binding protein of the synaptic vesicle membrane.},
  author={Hubert Rehm and Bertram Wiedenmann and Heinrich Betz},
  journal={The EMBO Journal},
Synaptophysin, a mol. wt 38 000 glycopolypeptide of the synaptic vesicle membrane, was solubilized using Triton X‐100 and purified by immunoaffinity or ion‐exchange chromatography. From gel permeation and sucrose‐density centrifugation in H2O/D2O, a Stokes radius of 7.3 nm, a partial specific volume of 0.830 and a total mol. wt of 119 000 were calculated for the native protein. Cross‐linking of synaptic vesicles with glutaraldehyde, dimethylsuberimidate, or Cu2+ ‐o‐phenantroline, resulted in… Expand
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