Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath).

@article{Cski2001MolecularCO,
  title={Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath).},
  author={R{\'o}bert Cs{\'a}ki and T{\'i}mea Hancz{\'a}r and Levente Bodrossy and John Colin Murrell and Korn{\'e}l L Kov{\'a}cs},
  journal={FEMS microbiology letters},
  year={2001},
  volume={205 2},
  pages={203-7}
}
The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A DeltahupSL deletion mutant of Mc. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo.