Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s.

  title={Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s.},
  author={David Mart{\'i}nez-Torres and Fabrice Chandre and Martin S Williamson and Fr{\'e}d{\'e}ric Darriet and Jean Baptiste Berg{\'e} and Alan L. Devonshire and Pierre Guillet and Nicole Pasteur and David Pauron},
  journal={Insect Molecular Biology},
Pyrethroid-impregnated bednets are playing an increasing role for combating malaria, especially in stable malaria areas. [] Key Result Using this test, we found this mutation in six out of seven field samples from West Africa, its frequency being closely correlated with survival to pyrethroid exposure. This diagnostic test should bring major improvement for field monitoring of pyrethroid resistance, within the framework of malaria control programmes.

Investigating knockdown resistance (kdr) mechanism against pyrethroids/DDT in the malaria vector Anopheles funestus across Africa

Analysis of patterns of genetic diversity of the VGSC gene revealed a high polymorphism level of this gene across Africa with no evidence of directional selection suggesting a limited role for knockdown resistance in pyrethroid resistance in An.

A simplified high-throughput method for pyrethroid knock-down resistance (kdr) detection in Anopheles gambiae

The HOLA assay, developed for detection of the kdr mutation, gives a bright blue colouration for a positive result whilst negative reactions remain colourless, making it suitable for use in resource-poor countries.

Pyrethroid resistance in the major malaria vector Anopheles arabiensis from Gwave, a malaria-endemic area in Zimbabwe

This study confirmed the presence of permethrin resistance in An.

Molecular evidence for a kdr‐like pyrethroid resistance mechanism in the malaria vector mosquito Anopheles stephensi

Molecular data is provided supporting the hypothesis that a kdr‐like pyrethroid‐resistance mechanism is present in An.

Identification of the knockdown resistance (Kdr) mutations in Anopheles gambiae s.l. in the Mouila area, Southwest Gabon

All the anopheline populations tested showed resistance to almost all insecticides except organophosphates and carbamates families, which remain the only lethal molecules.

Molecular evidence for historical presence of knock-down resistance in Anopheles albimanus, a key malaria vector in Latin America

Molecular evidence suggests the presence of kdr-type resistance in field-collected An.

Susceptibility to three pyrethroids and detection of knockdown resistance mutation in Ghanaian Anopheles gambiae sensu stricto

  • K. AdasiJ. Hemingway
  • Medicine, Biology
    Journal of vector ecology : journal of the Society for Vector Ecology
  • 2008
The relative ease in using molecular techniques has resulted in rapid detection of ostensible insecticide resistance genes in malaria vectors, but it is even more important to complement these molecular tools with routine insecticide testing in the field, especially if the insecticides are earmarked for public health use.

The genetic architecture of target‐site resistance to pyrethroid insecticides in the African malaria vectors Anopheles gambiae and Anopheles coluzzii

The results demonstrate that the molecular basis of target-site pyrethroid resistance in malaria vectors is more complex than previously appreciated, and provide a foundation for the development of new genetic tools to track the spread insecticides resistance and improve the design of strategies for insecticide resistance management.

Knockdown resistance (kdr) mutations in Indian Anopheles culicifacies populations

Two L1014-kdr mutations co-occurred in five populations belonging to Chhattisgarh and Odisha states of India and were found with very low allelic frequencies mostly in heterozygous condition and exhibited protection against deltamethrin.



Molecular studies of knockdown resistance to pyrethroids: cloning of domain II sodium channel gene sequences from insects

Knockdown resistance (kdr) is a target-site resistance mechanism that confers nerve insensitivity to DDT and pyrethroid insecticides. In the housefly, Musca domestica, molecular cloning of the

The molecular and population genetics of cyclodiene insecticide resistance.

The cyclodiene resistance gene Rdl (resistance to dieldrin) was cloned from a mutant of the model insect Drosophila resistant to cyclodienes and picrotoxinin and allowed for the design of a PCR based molecular monitoring technique.

Pyrethroid receptor in the insect Na sup + channel: Alteration of its properties in pyrethroid-resistant flies

It is concluded that linkage between pyrethroid and batrachotoxin binding sites is altered in the pyrethoid-resistant fly strains, probably due to a drastically decreased affinity of the Na{sup +} channel for pyrethroids.

The effectiveness of insecticide-impregnated bed nets in reducing cases of malaria infection: a meta-analysis of published results.

It is suggested that insecticide-impregnated bed nets are effective in preventing malaria, decreasing the incidence rate ratio by approximately 50% in field trials performed to date.

Mitochondrial Dna Variation in Rhopalosiphum padi (Homoptera: Aphididae) Populations from four Spanish Localities

The results show that the mitochondrial DNA methodology is a very promising tool for analyzing aphid population structure and for studying its relationships with the epidemiology of aphid-transmitted disease agents such as barley yellow dwarf virus.

Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila.

The deduced amino acid sequence of a Drosophila gene isolated with a vertebrate sodium channel complementary DNA probe revealed an organization virtually identical to the vertebrate sodium channel