Molecular characterization of plasmids encoding CTX-M-15 beta-lactamases from Escherichia coli strains in the United Kingdom.

@article{Karisik2006MolecularCO,
  title={Molecular characterization of plasmids encoding CTX-M-15 beta-lactamases from Escherichia coli strains in the United Kingdom.},
  author={Edi Karisik and Matthew J. Ellington and Rachel Pike and Rod E Warren and David Martin Livermore and Neil Woodford},
  journal={The Journal of antimicrobial chemotherapy},
  year={2006},
  volume={58 3},
  pages={665-8}
}
OBJECTIVES The UK, like other countries worldwide, has a growing problem with CTX-M beta-lactamase-producing Escherichia coli. Five major clonally related strains have been identified among CTX-M-15 producers. We characterize here the plasmids from clonal strains A and D. METHODS Plasmids were extracted and transformed into E. coli DH5alpha; conjugative mating was attempted on agar. MICs were determined by agar dilution. beta-Lactamases were typed by isoelectric focusing; antibiotic… CONTINUE READING

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