Molecular characterization of isolates of anagyris vein yellowing virus, plantago mottle virus and scrophularia mottle virus – comparison of various approaches for tymovirus classification

  title={Molecular characterization of isolates of anagyris vein yellowing virus, plantago mottle virus and scrophularia mottle virus – comparison of various approaches for tymovirus classification},
  author={R Koenig and C. W. A. Pleij and Dietrich E. Lesemann and Sandra Loss and H. Josef Vetten},
  journal={Archives of Virology},
Summary.The complete nucleotide sequences were determined for the genomic RNAs of three tymoviruses, i.e. isolates of anagyris vein yellowing virus (AVYV), plantago mottle virus (PlMoV) and scrophularia mottle virus (SrMV) which are all serologically closely related to ononis yellow mosaic virus (ibid) and to Nemesia ring necrosis virus (NeRNV), a recently described recombinant virus which is widely spread in commercially grown ornamental plant species belonging to the Scrophulariaceae. Total… 

Molecular characterization of a new tymovirus from Diascia ornamental plants

Two tymoviruses were identified in plants of Diascia × hybrida ‘Sun Chimes™ Coral’ that exhibited chlorotic mottling and reduced growth and sequence analysis revealed the presence of a new tymvirus, and the entire genomic sequence of this virus was determined.

Complete nucleotide sequence and experimental host range of Okra mosaic virus

Phylogenetic comparisons based on the RP, MP and CP amino acid sequences showed the close relationship of OkMV not only to other completely sequenced tymoviruses like Kennedya yellow mosaic virus (KYMV), Turnip yellow mosaicirus (TYMV) and Erysimum latent virus (ErLV), but also to Calopogonium yellow vein virus (CalYVV).

Nemesia ring necrosis virus: a new tymovirus with a genomic RNA having a histidylatable tobamovirus-like 3' end.

The complete nucleotide sequence of the genomic RNA of the new virus Nemesia ring necrosis virus (NeRNV), which is widespread in various ornamental plant species belonging to the Scrophulariaceae and

Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

The complete genomic nucleotide sequence of a new tymovirus species infecting chiltepin, the wild ancestor of Capsicum annuum pepper crops, in Mexico was determined, and its host range has been explored.



Characterization of a Tymovirus Isolated from Anagyris foetida as a Strain of Scrophularia Mottle Virus

A virus isolated from Anagyris foetida and infecting species in the Leguminosae and Solanaceae had typical properties of a tymovirus and induced the formation of double membrane-bounded invaginations in the chloroplasts of infected cells.


In immunodiffusion tests this virus proved to be serologically related with some tombusviruses, the closest being Grapevine Algerian latent virus (GALV), but showed wide molecular differences with all sequenced members of the genus.

Identification and Functional Analysis of the Turnip Yellow Mosaic Tymovirus Subgenomic Promoter

It is reported that the subgenomic promoter sequence of TYMV is located on a 494-nucleotide fragment, containing previously identified highly conserved sequence elements, shown here to be essential for promoter function.

Evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates

Criteria which have been suggested in addition to serology and sequence comparisons for tombusvirus species demarcation appear to be of little value for the classification of new tombusviruses.

Detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products.

Single-strand conformation polymorphism analysis was found to be a powerful tool for rapidly assigning large numbers of beet necrotic yellow vein virus (BNYVV) isolates to a known strain group as

Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae

The genome properties of three potexviruses which previously had been isolated from different genera in the family Cactaceae and had been found to be only distantly related serologically have been studied suggest that these viruses should be regarded as distinct virus species for which the names Zygocactus virus X, Schlumbergera virus X and Opuntia virus X are proposed.

A loop-structure in the serological classification system of tymoviruses.

The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses.

The 5'-terminal sequences of the virion protein mRNAs of ononis yellow mosaic and kennedya yellow mosaic tymoviruses were determined, and also the positions in the genomes of the transcription

A single missense mutation in the BR1 movement protein alters the host range of the squash leaf curl geminivirus.

A naturally occurring mutant of the SqLCV-R B component (BRtd1) which has lost the ability to infect N. benthamiana, but still retains full infectivity for pumpkin and squash is characterized.

Papain-like proteinase of turnip yellow mosaic virus: a prototype of a new viral proteinase group

It is speculated that all the tymo-like proteinase domains of the viral replicative proteins may share common biochemical and biological features.