Molecular characterization of inulosucrase from Leuconostoc citreum: a fructosyltransferase within a glucosyltransferase.

@article{OlivaresIllana2003MolecularCO,
  title={Molecular characterization of inulosucrase from Leuconostoc citreum: a fructosyltransferase within a glucosyltransferase.},
  author={Vanesa Olivares-Illana and Agust{\'i}n L{\'o}pez-Mungu{\'i}a and Clarita Olvera},
  journal={Journal of bacteriology},
  year={2003},
  volume={185 12},
  pages={3606-12}
}
The gene coding for inulosucrase in Leuconostoc citreum CW28, islA, was cloned, sequenced, and expressed in Escherichia coli. The recombinant enzyme catalyzed inulin synthesis from sucrose like the wild-type enzyme. Inulosucrase presents an unusual structure: its N-terminal region is similar to the variable region of glucosyltransferases, its catalytic domain is similar to fructosyltransferases from various microorganisms, and its C-terminal domain presents similarity to the glucan binding… CONTINUE READING
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