Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress

  title={Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress},
  author={Joaqu{\'i}n Espartero and Inmaculada S{\'a}nchez-Aguayo and Jos{\'e} Manuel Pardo},
  journal={Plant Molecular Biology},
A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and-II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 49.4% and 58.5% identity with glyoxalase-I isolated from bacteria and human, respectively. Furthermore, yeast cells expressing GLX1 showed a glyoxalase-I specific activity 20-fold… CONTINUE READING
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