Molecular characterization of enterohemorrhagic Escherichia coli O157:H7 isolates dispersed across Japan by pulsed-field gel electrophoresis and multiple-locus variable-number tandem repeat analysis.

@article{Pei2008MolecularCO,
  title={Molecular characterization of enterohemorrhagic Escherichia coli O157:H7 isolates dispersed across Japan by pulsed-field gel electrophoresis and multiple-locus variable-number tandem repeat analysis.},
  author={Yingxin Pei and Jun Terajima and Yasunori Saito and Reiko Suzuki and Nobuko Takai and Hidemasa Izumiya and Tomoko Morita-Ishihara and Makoto Ohnishi and Masashi Miura and Sunao Iyoda and Jiro Mitobe and Binyou Wang and Haruo Watanabe},
  journal={Japanese journal of infectious diseases},
  year={2008},
  volume={61 1},
  pages={
          58-64
        }
}
We identified seven distinct subtypes of enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were derived from sporadic cases and outbreaks from multiple prefectures in Japan in 2005. A surveillance system utilizing pulsed-field gel electrophoresis (PFGE), PulseNet Japan, was used. Some strains showed indistinguishable PFGE patterns using another restriction enzyme (BlnI or SpeI) in each subtype of EHEC O157:H7 isolates that were routinely subtyped by the XbaI PFGE pattern. In order… CONTINUE READING

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