Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence.

@article{DeShazer1994MolecularCO,
  title={Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence.},
  author={David DeShazer and Gwendolyn E. Wood and Richard L. Friedman},
  journal={Molecular microbiology},
  year={1994},
  volume={14 1},
  pages={123-30}
}
In this report we evaluate the role of catalase in the survival of Bordetella pertussis within human polymorphonuclear leukocytes (PMNs). Crude extracts of B. pertussis exhibited a single catalase activity when subjected to non-denaturing polyacrylamide gel electrophoresis and assayed for catalase activity. A plasmid containing B. pertussis katA was identified by complementation of UM255, a catalase-deficient strain of Escherichia coli. The nucleotide sequence of katA predicts a 55 kDa protein… CONTINUE READING

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