Molecular characterization of cDNA encoding resistance gene-like sequences in Buchloe dactyloides

@article{Budak2006MolecularCO,
  title={Molecular characterization of cDNA encoding resistance gene-like sequences in Buchloe dactyloides},
  author={Hikmet Budak and Zeynep Kasap and R. C. Shearman and Ismail M Dweikat and Ugur Sezerman and Aslam Mahmood},
  journal={Molecular Biotechnology},
  year={2006},
  volume={34},
  pages={293-301}
}
Current knowledge of resistance (R) genes and their use for genetic improvement in buffalograss (Buchloe dactyloides [Nutt.] Engelm.) lag behind most crop plants. This study was conducted to clone and characterize cDNA encoding R gene-like (RGL) sequences in buffalograss. This report is the first to clone and characterize of buffalograss RGLs. Degenerate primers designed from the conserved motifs of known R genes were used to amplify RGLs and fragments of expected size were isolated and cloned… CONTINUE READING
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