Molecular characterization of beta-lactamase inclusion bodies produced in Escherichia coli. 1. Composition.

@article{Valax1993MolecularCO,
  title={Molecular characterization of beta-lactamase inclusion bodies produced in Escherichia coli. 1. Composition.},
  author={Pascal Valax and George D. Georgiou},
  journal={Biotechnology progress},
  year={1993},
  volume={9 5},
  pages={
          539-47
        }
}
We have determined the macromolecular composition of inclusion bodies formed by overexpressing beta-lactamase from three different expression systems as a function of the growth conditions. The inclusion bodies were purified by differential gradient centrifugation and detergent extraction. Both the expression system and the growth conditions were shown to have a pronounced effect on inclusion body composition. Specifically, contaminating polypeptides ranged from less than 5% to over 50% of the… CONTINUE READING
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