Molecular characterization of a phosphatidylcholine-hydrolyzing phospholipase C.

@article{Preuss2001MolecularCO,
  title={Molecular characterization of a phosphatidylcholine-hydrolyzing phospholipase C.},
  author={I Preuss and Iris Kaiser and Ulrich Gehring},
  journal={European journal of biochemistry},
  year={2001},
  volume={268 19},
  pages={5081-91}
}
While searching for a phospholipase C (PLC) specific for phosphatidylcholine in mammalian tissues, we came across such an activity originating from a contamination of Pseudomonas fluorescens. This psychrophilic bacterium was found to contaminate placental extracts upon processing in the cold. The secreted phosphatidylcholine-hydrolyzing PLC was purified by a combination of chromatographic procedures. As substrates, the enzyme preferred dipalmitoyl-phosphatidylcholine and 1-palmitoyl-2… CONTINUE READING

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