Molecular characterization of a glucose-inhibited division gene, gidA, that regulates cytotoxic enterotoxin of Aeromonas hydrophila.

@article{Sha2004MolecularCO,
  title={Molecular characterization of a glucose-inhibited division gene, gidA, that regulates cytotoxic enterotoxin of Aeromonas hydrophila.},
  author={Jian Sha and Elena V. Kozlova and Amin A. Fadl and Juan Pablo Olano and Clifford W. Houston and Johnny W. Peterson and Ashok Kumar Chopra},
  journal={Infection and immunity},
  year={2004},
  volume={72 2},
  pages={1084-95}
}
By using a mini-transposon, we obtained two mutated strains of a diarrheal isolate, SSU, of Aeromonas hydrophila that exhibited a 50 to 53% reduction in the hemolytic activity and 83 to 87% less cytotoxic activity associated with the cytotoxic enterotoxin (Act). Act is a potent virulence factor of A. hydrophila and has been shown to contribute significantly to the development of both diarrhea and septicemia in animal models. Subsequent cloning and DNA sequence analysis revealed that transposon… CONTINUE READING