Molecular characterization of a gene that confers 2‐deoxyglucose resistance in yeast

@article{Sanz1994MolecularCO,
  title={Molecular characterization of a gene that confers 2‐deoxyglucose resistance in yeast},
  author={Pascual Sanz and Francisca Randez-Gil and Jose A Prieto},
  journal={Yeast},
  year={1994},
  volume={10}
}
We have isolated a gene whose expression enables yeast cells to overcome the inhibition of growth produced by the presence of 2‐deoxyglucose. The gene contains an open reading frame of 738 bp that may code for a protein of 27 100 Da. Cells carrying this gene contain high levels of a specific 2‐deoxyglucose‐6‐phosphate phosphatase. The expression of this phosphatase is increased by the presence of 2‐deoxyglucose and is constant along the growth curve. The sequence reported here has the GenBank… 
The expression of a specific 2-deoxyglucose-6P phosphatase prevents catabolite repression mediated by 2-deoxyglucose in yeast
TLDR
It was found that in DOGR yeasts, 2-DOG was not able to trigger glucose repression, even at concentrations of 0.5%.
Identification of nuclear genes affecting 2-Deoxyglucose resistance in Schizosaccharomyces pombe
TLDR
Findings suggest that mechanisms involved in 2-DG resistance differ between budding and fission yeasts, and odr1, encoding an uncharacterized hydrolase, led to strong resistance and altered invertase expression when overexpressed.
2-Deoxyglucose resistance: a novel selection marker for plant transformation
TLDR
The acceptability of the resistance gene derived from baker's yeast, the unobjectionable toxicological data of 2-DOG as well as the normal phenotype of DOGR1-expressing plants support the use of this selection system in crop plant transformation.
DOGR1 and DOGR2: Two genes from Saccharomyces cerevisiae that confer 2‐deoxyglucose resistance when overexpressed
Saccharomyces cerevisiae contains two genes (DOGR1 and DOGR2) that are able to confer 2‐deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the
Cooperative Regulation of DOG2, Encoding 2-Deoxyglucose-6-Phosphate Phosphatase, by Snf1 Kinase and the High-Osmolarity Glycerol–Mitogen-Activated Protein Kinase Cascade in Stress Responses of Saccharomyces cerevisiae
TLDR
The results suggest that Snf1p kinase and the high-osmolarity glycerol-mitogen-activated protein kinase cascade are likely to be involved in the signaling pathway of oxidative stress and osmotic stress in regulation of DOG2.
Lactobacillus curvatus has a glucose transport system homologous to the mannose family of phosphoenolpyruvate-dependent phosphotransferase systems.
In Lactobacillus curvatus, a phosphoenolpyruvate:mannose phosphotransferase system (mannose-PTS) has been characterized and it was shown to be involved in glucose and mannose transport, but no
The induction of HAD-like phosphatases by multiple signaling pathways confers resistance to the metabolic inhibitor 2-deoxyglucose
TLDR
Results show that HAD-like phosphatases are evolutionarily conserved regulators of 2DG resistance and suggest that cancer cells with increased abundance of this phosphatase could escape the toxic effects of2DG.
Purification and Characterization of Two Isoenzymes of DL-Glycerol-3-phosphatase from Saccharomyces cerevisiae
TLDR
Together with DOG1 and DOG2, encoding two highly homologous enzymes that dephosphorylate 2-deoxyglucose-6-phosphate, GPP1 and GPP2 constitute a new family of genes for low molecular weight phosphatases.
Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiae
TLDR
Signalling mutants, either in the cAMP‐dependent protein kinase A pathway or in aprotein kinase cascade, have been analyzed during osmotic stress via 2‐D PAGE, grouping proteins/genes apparently regulated via similar mechanismus.
...
...

References

SHOWING 1-10 OF 19 REFERENCES
Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency
We have found that Saccharomyces cerevisiae acquires spontaneously increasing resistance to 2-deoxyglucose at a very high frequency. This finding allows the easy isolation of different types of
Mutational analysis of a yeast transcriptional terminator
TLDR
A DNA fragment from Saccharomyces cerevisiae that specifies mRNA 3' end formation for the convergently transcribed CYC1 and UTR1 genes is isolated and mutagenized and suggests that the fragment contains the sequences of two, unidirectional terminator elements.
One-step gene disruption in yeast.
Distinct cis‐acting signals enhance 3′ endpoint formation of CYC1 mRNA in the yeast Saccharomyces cerevisiae.
TLDR
The production of 3′ termini of yeast mRNA may involve at least two functionally distinct elements working in concert, which determines the sites of preferred 3′ mRNA termini, as represented by the cyc1–512 termini.
Primary structure of Saccharomyces cerevisiae NADPH-cytochrome P450 reductase deduced from nucleotide sequence of its cloned gene.
TLDR
The possible functional domains related to binding of FAD, FMN, and NADPH were well conserved among all five species compared and showed 33-34% similarity with those of the rat, rabbit, pig, and trout reductases.
...
...