Molecular characterization of a gene that confers 2‐deoxyglucose resistance in yeast

  title={Molecular characterization of a gene that confers 2‐deoxyglucose resistance in yeast},
  author={Pascual Sanz and Francisca Randez-Gil and Jose A Prieto},
We have isolated a gene whose expression enables yeast cells to overcome the inhibition of growth produced by the presence of 2‐deoxyglucose. The gene contains an open reading frame of 738 bp that may code for a protein of 27 100 Da. Cells carrying this gene contain high levels of a specific 2‐deoxyglucose‐6‐phosphate phosphatase. The expression of this phosphatase is increased by the presence of 2‐deoxyglucose and is constant along the growth curve. The sequence reported here has the GenBank… 
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Cooperative Regulation of DOG2, Encoding 2-Deoxyglucose-6-Phosphate Phosphatase, by Snf1 Kinase and the High-Osmolarity Glycerol–Mitogen-Activated Protein Kinase Cascade in Stress Responses of Saccharomyces cerevisiae
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Lactobacillus curvatus has a glucose transport system homologous to the mannose family of phosphoenolpyruvate-dependent phosphotransferase systems.
In Lactobacillus curvatus, a phosphoenolpyruvate:mannose phosphotransferase system (mannose-PTS) has been characterized and it was shown to be involved in glucose and mannose transport, but no
The induction of HAD-like phosphatases by multiple signaling pathways confers resistance to the metabolic inhibitor 2-deoxyglucose
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Together with DOG1 and DOG2, encoding two highly homologous enzymes that dephosphorylate 2-deoxyglucose-6-phosphate, GPP1 and GPP2 constitute a new family of genes for low molecular weight phosphatases.
Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiae
Signalling mutants, either in the cAMP‐dependent protein kinase A pathway or in aprotein kinase cascade, have been analyzed during osmotic stress via 2‐D PAGE, grouping proteins/genes apparently regulated via similar mechanismus.


Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency
We have found that Saccharomyces cerevisiae acquires spontaneously increasing resistance to 2-deoxyglucose at a very high frequency. This finding allows the easy isolation of different types of
Mutational analysis of a yeast transcriptional terminator
A DNA fragment from Saccharomyces cerevisiae that specifies mRNA 3' end formation for the convergently transcribed CYC1 and UTR1 genes is isolated and mutagenized and suggests that the fragment contains the sequences of two, unidirectional terminator elements.
One-step gene disruption in yeast.
Distinct cis‐acting signals enhance 3′ endpoint formation of CYC1 mRNA in the yeast Saccharomyces cerevisiae.
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