Molecular characterization of a cDNA encoding putative vitellogenin from the Pacific oyster Crassostrea gigas.

@article{Matsumoto2003MolecularCO,
  title={Molecular characterization of a cDNA encoding putative vitellogenin from the Pacific oyster Crassostrea gigas.},
  author={Toshie Matsumoto and Akihumi M Nakamura and Katsuyoshi Mori and Toshiaki Kayano},
  journal={Zoological science},
  year={2003},
  volume={20 1},
  pages={37-42}
}
To elucidate the molecular mechanisms involved in oogenesis, we applied a differential display method to identify genes whose expression was detected only in ovaries containing oocytes. One of the cDNA fragments isolated by mRNA differential display was similar in structure to vitellogenin. Using this fragment, a full-length cDNA encoding putative vitellogenin in the Pacific oyster Crassostrea gigas was cloned by RACE (rapid amplification of cDNA ends), and its amino acid sequence was deduced… CONTINUE READING
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