Molecular characterization of a cDNA clone encoding the Epstein-Barr virus (EBV) DNase.

@article{Chen1990MolecularCO,
  title={Molecular characterization of a cDNA clone encoding the Epstein-Barr virus (EBV) DNase.},
  author={M R Chen and T Y Hsu and Jin Yun Helen Chen and Chung S Yang},
  journal={Journal of virological methods},
  year={1990},
  volume={29 2},
  pages={127-41}
}
RNA from IdUrd-treated P3HR1 cells was used for the construction of a cDNA library and screened with B95-8 EBV DNA BamHI fragment B and G probes. One clone, BG9, containing a 1.7 kb cDNA insert was further studied. Complete DNA sequence analysis revealed that BG9 encompassed the B95-8 EBV DNA sequences from nucleotide 120,747 to nucleotide 122,412 and corresponded to the BGLF5 open reading frame of the EBV DNase gene. Comparison of the sequences of BG9 with that of published B95-8 EBV DNA… CONTINUE READING