Molecular characterization of Vibrio cholerae isolates from Iran 2012 and 2013 outbreaks
@article{Bakhshi2016MolecularCO, title={Molecular characterization of Vibrio cholerae isolates from Iran 2012 and 2013 outbreaks}, author={Bita Bakhshi}, journal={Letters in Applied Microbiology}, year={2016}, volume={62} }
The aim of this study was to assess the genetic diversity of Vibrio cholerae isolated from 2012 and 2013 outbreaks in Iran, with regard to their virulence properties. A total of 20 V. cholerae strains were collected from Sistan‐Baluchestan province of Iran during 2012 and 2013 outbreaks. Hybridization assays showed the presence of ctx, zot, ace and rstC genes related to CTX and RS1 phages in all of the isolates. PCR assay indicated the concomitant presence of ORFs within RTX (1448, 1451) and…
3 Citations
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References
SHOWING 1-10 OF 31 REFERENCES
Emergence of Vibrio cholerae O1 classical biotype in 2012 in Iran
- BiologyLetters in applied microbiology
- 2014
Investigation of V. cholerae isolates from recent outbreak in Iran and to characterize the ctxB allelic sequence of isolates demonstrated diversity in virulence gene content of strains with identical PFGE patterns and provided evidences of the import of a V. Cholerae strain with classical biotype from out of the authors' country.
Clonal Dissemination of a Single Vibrio cholerae O1 Biotype El Tor Strain in Sistan–Baluchestan Province of Iran During 2013
- BiologyCurrent Microbiology
- 2015
Although much is known about the mechanisms affecting cholera spread, cholera outbreaks occur annually in Iran. The aim of this study was to characterize and assess the clonal correlation of strains…
Comparative PCR-based fingerprinting of Vibrio cholerae isolated in Malaysia.
- Biology, MedicineThe Journal of general and applied microbiology
- 2011
A Vibrio cholerae Repeats-PCR approach which targets the genetic polymorphism of the integron island of Vibrios was used and compared with other PCR-based fingerprinting methods in subtyping to overcome the inadequacy of any one approach.
Comparison of distribution of virulence determinants in clinical and environmental isolates of Vibrio cholera.
- Biology, MedicineIranian biomedical journal
- 2008
The absence of all virulence determinants in the environmental strains may suggest that certain ecological features must be present for V. cholerae to acquire a complete set of virulent determinants and to turn them into pathogenic strains.
Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia.
- BiologyJournal of medical microbiology
- 1997
A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) and by ribotyping, and all isolates were positive for ctx A and zot, which was confirmed by hybridisation.
Evaluation of Typing of Vibrioparahaemolyticus by Three PCR Methods Using Specific Primers
- BiologyJournal of Clinical Microbiology
- 2001
Three rapid PCR typing methods for this pathogen are designed and evaluated using primers designed on the basis of the following specific sequences: conserved ribosomal gene spacer sequence (RS), repetitive extragenic palindromic sequence (REP), and enterobacterial repetitive intergenic consensus sequence (ERIC).
Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis
- BiologyJournal of clinical microbiology
- 1994
Pulsed-field gel electrophoresis was more discriminating than other previously described subtyping assays for V. cholerae O1 and appears to be a useful epidemiologic tool.
Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains
- BiologyApplied and environmental microbiology
- 1995
It is concluded from the results of this study that it is possible to detect an emerging virulent strain in a cholera-endemic region and ERIC-PCR constitutes a powerful tool for determination of the virulence potential of V. cholerae O1 strains isolated in surveillance programs and for molecular epidemiological investigations.
Virulence Genes in Environmental Strains ofVibrio cholerae
- BiologyApplied and Environmental Microbiology
- 2000
This study demonstrates the presence and expression of critical virulence genes or their homologues in diverse environmental strains of V. cholerae, which appear to constitute an environmental reservoir of virulent genes, thereby providing new insights into the ecology ofV.
Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns.
- BiologyResearch in microbiology
- 1990