Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis

@article{Goswami2009MolecularCO,
  title={Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis},
  author={L. Goswami and P. Prasad and V. Tandon and A. Chatterjee},
  journal={Parasitology Research},
  year={2009},
  volume={104},
  pages={1485-1490}
}
Gastrodiscoides hominis (Digenea: Paramphistomata: Gastrodiscidae) is an amphistomid intestinal fluke of pigs causing gastrodiscoidiosis. With the use of molecular tools assisting the conventional diagnostic procedures, we aimed at finding out molecular characterization of G. hominis using PCR amplifications of rDNA ITS (1, 2) sequences. The sequences obtained (GenBank accession numbers EF027096, EF027097, EF027098, EU887294, and EU887295) were compared with available sequences of other… Expand
Multilocus sequence evaluation for differentiating species of the trematode Family Gastrothylacidae, with a note on the utility of mitochondrial COI motifs in species identification.
TLDR
In sequence and phylogenetic analyses the COI gene turned out to be the most useful marker in identifying the gastrothylacid species, with the exception of Gastrothylax crumenifer, which showed a high degree of intraspecific variations among its isolates. Expand
Utilizing ribosomal DNA gene marker regions to characterize the metacercariae (Trematoda: Digenea) parasitizing piscine intermediate hosts in Manipur, Northeast India
TLDR
Molecular techniques utilizing PCR amplification of rDNA regions of larger subunit, smaller subunit and inter transcribed spacers and the rDNA ITS2 secondary structure provided additional confirmation of the robustness of the molecular marker as a tool for taxon-specific characterization. Expand
New approach to molecular characterization of Paramphistomum cervi and Carmyerius gregarius and comparative analyses with selected trematodes
TLDR
This work will give a useful guide for other researchers for the molecular taxonomic position of Paramphistomatidae spp. Expand
Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae).
TLDR
This first report of molecular characterization for P. leydeni improves the current knowledge of the genus and establishes precedents for further specimen classifications. Expand
Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region
The nodular tapeworm, Raillietina echinobothrida is a well studied avian gastrointestinal parasite of family Davaineidae (Cestoda: Cyclophyllidea). It is reported to be the largest in size and secondExpand
Utility of divergent domains of 28S ribosomal RNA in species discrimination of paramphistomes (Trematoda: Digenea: Paramphistomoidea)
TLDR
28S (D1–D3) rRNA provided a significant resolution of the taxa corroborating with the taxonomy of these flukes and thus proved to be more robust as a phylogenetic marker for lower levels than 18S rRNA. Expand
Loads of trematodes: discovering hidden diversity of paramphistomoids in Kenyan ruminants
TLDR
Paramphistomoids of domestic ruminants provide one of the most abundant sources of parasitic flatworm biomass, and because of the predilection of several species use Bulinus and Biomphalaria snail hosts, have interesting linkages with the biology of animal and human schistosomes to in Africa. Expand
A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region
TLDR
IT region is more reliable and precise marker for demarcation and identification of species in combination of other DNA markers, and will lead to explore the untouched areas of trematodes. Expand
A molecular approach for identification of paramphistomes from Africa and Asia.
TLDR
The results confirm that ITS2 is a good molecular marker for paramphistome identification and can be used to determine the affiliation of samples among the different taxa of Paramphistomoidea. Expand
Worms of Humans
This chapter defines the group of parasitic worms, which belong to the main groups of trematodes, cestodes, nematodes, pentastomids, acanthocephalans and leeches. Important species of respectiveExpand
...
1
2
...

References

SHOWING 1-10 OF 40 REFERENCES
Use of ITS rDNA for discriminating of larval stages of two microphallid (Digenea) species using Hydrobia ulvae (Pennant, 1777) and Corophium volutator (Pallas, 1766) as intermediate hosts
TLDR
The results support the view that the two types of microphallid trematode Maritrema subdolum actually represent different species, and these primers proved to be efficient diagnostic tools for identifying single larval-stage individuals. Expand
Evolutionary biology of parasitic platyhelminths: The role of molecular phylogenetics.
TLDR
The ways in which molecular data are helping us recognize the various lineages of flatworms are discussed, including ultrastructural evidence and molecular data from nucleic acid sequences. Expand
PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA of giant intestinal fluke, Fasciolopsis buski (Lankester, 1857) Looss, 1899
TLDR
The results corroborate that the ITS sequences are not stage specific and are conserved through different stages of development of the fluke, and thus could be used as species markers. Expand
Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers.
TLDR
The three species of amphistomes were identified based on the difference in the restriction sites of Acc II on the ITS2 sequence, and a PCR-RFLP technique based on ITS2 sequences for the species identification in single eggs of the parasites was evaluated. Expand
Molecular phylogeographic studies on Paragonimus westermani in Asia.
TLDR
It is considered that the populations sampled can be divided into two species, one in north-east Asia and the other in south Asia, and three or four groups within P. westermani are found. Expand
A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Pagumogonimus Chen.
The status of the genera Euparagonimus Chen, 1963 and Pagumogonimus Chen, 1963 relative to Paragonimus Braun, 1899 was investigated using DNA sequences from the mitochondrial cytochrome c oxidaseExpand
A molecular phylogeny of the human schistosomes.
TLDR
Both mitochondrial and nuclear DNA data strongly support "traditional" hypotheses that members of the Schistosoma haematobium group form a monophyletic clade, and the African schistosomes form a clade to the exclusion of the two Asian species. Expand
THE PROBLEM OF GASTRODISCOIDES HOMINIS (LEWIS AND MCCONNELL, 1876) LEIPER, 1913.
  • J. Buckley
  • Biology, Medicine
  • Journal of helminthology
  • 1964
Gastrodiscoides hominis is unique among the helminthic infections of man in that it is the only common representative of the Amphistome group in the human host; Watsonius watsoni (Conyingham, 1904),Expand
Surface fine topography and PCR-based determination of metacercaria of Paragonimus sp. from edible crabs in Arunachal Pradesh, Northeast India
TLDR
The surface topography including the number and distribution of papillae and spination patterns and the ITS2 sequences of the metacercariae strongly suggest that the Paragonimus species, prevalent in the region of India, is in fact P. westermani. Expand
Plant-Borne Trematode Zoonoses: Fascioliasis and Fasciolopsiasis
There are six plant-borne trematode species known affecting humans: Fasciola hepatica, F. gigantica, and Fasciolopsis buski (Fasciolidae), Gastrodiscoides hominis (Gastrodiscidae), Watsonius watsoni,Expand
...
1
2
3
4
...