Molecular characterization and mutational screening of the PRKAG3 gene in the horse

  title={Molecular characterization and mutational screening of the PRKAG3 gene in the horse},
  author={H.-B. Park and Stefan Marklund and Jin Tae Jeon and James R. Mickelson and Stephanie J. Valberg and Kaj Sandberg and L. Andersson},
  journal={Cytogenetic and Genome Research},
  pages={211 - 216}
The PRKAG3 gene encodes a muscle-specific isoform of the regulatory γ subunit of AMP-activated protein kinase (AMPK). A major part of the coding PRKAG3 sequence was isolated from horse muscle cDNA using reverse-transcriptase (RT)-PCR analysis. Horse-specific primers were used to amplify genomic fragments containing 12 exons. Comparative sequence analysis of horse, pig, mouse, human, Fugu, and zebrafish was performed to establish the exon/intron organization of horse PRKAG3 and to study the… 

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