Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus.

@article{Berka1998MolecularCA,
  title={Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus.},
  author={Randy M. Berka and Michael Rey and Kimberly Lara Brown and Tony Byun and Alan V Klotz},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 11},
  pages={4423-7}
}
The phyA gene encoding an extracellular phytase from the thermophilic fungus Thermomyces lanuginosus was cloned and heterologously expressed, and the recombinant gene product was biochemically characterized. The phyA gene encodes a primary translation product (PhyA) of 475 amino acids (aa) which includes a putative signal peptide (23 aa) and propeptide (10 aa). The deduced amino acid sequence of PhyA has limited sequence identity (ca. 47%) with Aspergillus niger phytase. The phyA gene was… CONTINUE READING

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Polypeptides having phytase activity and nucleic acids encoding same

R. M. Berka, M. W. Rey, A. V. Klotz
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