Molecular characteristics of mammalian and insect amino acid transporters: implications for amino acid homeostasis.

@article{Castagna1997MolecularCO,
  title={Molecular characteristics of mammalian and insect amino acid transporters: implications for amino acid homeostasis.},
  author={Michela Castagna and Chairat Shayakul and D Trotti and Vellea Franca Sacchi and William R. Harvey and Matthias A. Hediger},
  journal={The Journal of experimental biology},
  year={1997},
  volume={200 Pt 2},
  pages={269-86}
}
In mammalian cells, the uptake of amino acids is mediated by specialized, energy-dependent and passive transporters with overlapping substrate specificities. Most energy-dependent transporters are coupled either to the cotransport of Na+ or Cl- or to the countertransport of K+. Passive transporters are either facilitated transporters or channels. As a prelude to the molecular characterization of the different classes of transporters, we have isolated transporter cDNAs by expression-cloning with… CONTINUE READING

4 Figures & Tables

Connections & Topics

Mentioned Connections BETA
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
Amino AcidsChemical structure ofGlutamic Acid
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
For example , in the central nervous system , glutamate transporters are critically important in maintaining the extracellular glutamate concentration below neurotoxic levels , and defects of the human D2 gene have been shown to account for the formation of kidney stones in patients with cystinuria .
For example , in the central nervous system , glutamate transporters are critically important in maintaining the extracellular glutamate concentration below neurotoxic levels , and defects of the human D2 gene have been shown to account for the formation of kidney stones in patients with cystinuria .
Recent data showed that injection of mRNA prepared from the midgut of Manduca sexta into Xenopus laevis oocytes induced a 1.5- to 2.5-fold stimulation of the Na(+)-dependent uptake of both leucine and phenylalanine ( 0.2 mmoll-1 , pH 8) .
Recent data showed that injection of mRNA prepared from the midgut of Manduca sexta into Xenopus laevis oocytes induced a 1.5- to 2.5-fold stimulation of the Na(+)-dependent uptake of both leucine and phenylalanine ( 0.2 mmoll-1 , pH 8) .
PotassiumNo subtypeSodium
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
Most energy - dependent transporters are coupled either to the cotransport of Na+ or Cl- or to the countertransport of K+ .
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
SodiumNo subtypePotassium
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
Most energy - dependent transporters are coupled either to the cotransport of Na+ or Cl- or to the countertransport of K+ .
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
In addition to K+ , Na+ can also be coupled to amino acid uptake at lower pH , but the Na+/K+ ratio of the hemolymph is so low that K+ is probably the major coupling ion in vivo .
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
Mammalian transporters investigated include the dibasic and neutral amino acid transport protein D2/NBAT ( system b0 + ) and the Na(+)- and K(+)-dependent neuronal and epithelial high - affinity glutamate transporter EAAC1 ( system XAG- ) .
All Topics