Molecular chaperones (TrxA, SUMO, Intein, and GST) mediating expression, purification, and antimicrobial activity assays of plectasin in Escherichia coli.

@article{Chen2015MolecularC,
  title={Molecular chaperones (TrxA, SUMO, Intein, and GST) mediating expression, purification, and antimicrobial activity assays of plectasin in Escherichia coli.},
  author={Xin Chen and Jiawei Shi and Rongchang Chen and Yaoan Wen and Yu Shi and Zhe Zhu and Songwen Guo and Gangyi Yang},
  journal={Biotechnology and applied biochemistry},
  year={2015},
  volume={62 5},
  pages={606-14}
}
Plectasin (PS) is the first defensin to be isolated from a fungus, the saprophytic ascomycete Pseudoplectania nigrella, and active against Streptococcus pneumoniae and S. aureus, including antibiotic-resistant pathogens. To establish a bacterium-based production system, we compared the efficiency of four molecular chaperones and corresponding cleavage to the expression and purification of plectasin. The results showed that the yield of plectasin combined with thioredoxin A (TrxA) and small… CONTINUE READING