Molecular breeding of transgenic rice expressing a xylanase domain of the xynA gene from Clostridium thermocellum

@article{Kimura2003MolecularBO,
  title={Molecular breeding of transgenic rice expressing a xylanase domain of the xynA gene from Clostridium thermocellum},
  author={Toshiyuki Kimura and Tomomi Mizutani and Tsutomu Tanaka and Takayoshi Koyama and Kazuo Sakka and Kunio Ohmiya},
  journal={Applied Microbiology and Biotechnology},
  year={2003},
  volume={62},
  pages={374-379}
}
The gene encoding the catalytic domain of thermostable xylanase from Clostridium thermocellum F1 was expressed in rice plants under the control of a constitutive promoter. The gene encoding Xylanase A was modified to encode the catalytic domain of family 11 xylanase without the signal sequence (xynA1), and was introduced into rice plants and expressed under the control of a modified cauliflower mosaic virus 35S promoter. Zymogram analysis indicated that the recombinant xylanase was produced in… CONTINUE READING