Molecular breeding of the basidiomycete Coprinus cinereus strains with high lignin-decolorization and -degradation activities using novel heterologous protein expression vectors

@article{Ogawa1998MolecularBO,
  title={Molecular breeding of the basidiomycete Coprinus cinereus strains with high lignin-decolorization and -degradation activities using novel heterologous protein expression vectors},
  author={Keiko Ogawa and Takashi Yamazaki and Takeshi Hasebe and Susumu Kajiwara and Akiko Watanabe and Yasuhiko Asada and Kazuo Shishido},
  journal={Applied Microbiology and Biotechnology},
  year={1998},
  volume={49},
  pages={285-289}
}
Two chromosome-integrating vectors, pLC1 and pLC2, were used. The former is the pUC19-based vector carrying the Lentinus edodes ras gene promoter and priA gene terminator, and the latter is the pBR322-based vector carrying the promoter and terminator of the priA gene. The manganese (II) peroxidase (MnP) cDNA (mnpc) derived from Pleurotus ostreatus was fused between the promoter and terminator of pLC1 and pLC2, yielding the recombinant plasmids pLC1-mnp and pLC2-mnp. These plasmids were… CONTINUE READING

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