Molecular breeding of carotenoid biosynthetic pathways

@article{SchmidtDannert2000MolecularBO,
  title={Molecular breeding of carotenoid biosynthetic pathways},
  author={Claudia Schmidt-Dannert and Daisuke Umeno and Frances H. Arnold},
  journal={Nature Biotechnology},
  year={2000},
  volume={18},
  pages={750-753}
}
The burgeoning demand for complex, biologically active molecules for medicine, materials science, consumer products, and agrochemicals is driving efforts to engineer new biosynthetic pathways into microorganisms and plants. We have applied principles of breeding, including mixing genes and modifying catalytic functions by in vitro evolution, to create new metabolic pathways for biosynthesis of natural products in Escherichia coli. We expressed shuffled phytoene desaturases in the context of a… 
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