Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase.

@article{Qian2005MolecularBO,
  title={Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase.},
  author={Minxie Qian and El Hassan Ajandouz and Françoise Payan and Virginie Nahoum},
  journal={Biochemistry},
  year={2005},
  volume={44 9},
  pages={3194-201}
}
Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with maltopentaose using a chloride-free… CONTINUE READING