Molecular basis of estrogen-induced cyclooxygenase type 1 upregulation in endothelial cells.

@article{Gibson2005MolecularBO,
  title={Molecular basis of estrogen-induced cyclooxygenase type 1 upregulation in endothelial cells.},
  author={Linda L. Gibson and Lisa D. Hahner and Sherri Osborne-Lawrence and Zohre German and Kenneth K. Wu and Ken L. Chambliss and Philip W. Shaul},
  journal={Circulation research},
  year={2005},
  volume={96 5},
  pages={518-25}
}
Estrogen upregulates cyclooxygenase-1 (COX-1) expression in endothelial cells. To determine the basis of this process, studies were performed in ovine endothelial cells transfected with the human COX-1 promoter fused to luciferase. Estradiol (E2) caused activation of the COX-1 promoter with maximal stimulation at 10(-8) mol/L E2, and the response was mediated by either ERalpha or ERbeta. Mutagenesis revealed a primary role for a putative Sp1 binding motif at -89 (relative to the ATG codon) and… CONTINUE READING