Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans.

@article{Paschinger2004MolecularBO,
  title={Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans.},
  author={Katharina Paschinger and Dubravko Rendi{\'c} and Guenter Lochnit and Verena Jantsch and Iain B H Wilson},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 48},
  pages={49588-98}
}
Cross-reactivity with anti-horseradish peroxidase antiserum is a feature of many glycoproteins from plants and invertebrates; indeed staining with this reagent has been used to track neurons in Drosophila melanogaster and Caenorhabditis elegans. Although in insects the evidence indicates that the cross-reaction results from the presence of core alpha1,3-fucosylated N-glycans, the molecular basis for anti-horseradish peroxidase staining in nematodes has been unresolved to date. By using Western… CONTINUE READING