Molecular basis for the ribosome functioning as an L-tryptophan sensor.

  title={Molecular basis for the ribosome functioning as an L-tryptophan sensor.},
  author={Lukas Bischoff and Otto Berninghausen and Roland Beckmann},
  journal={Cell reports},
  volume={9 2},
Elevated levels of the free amino acid L-tryptophan (L-Trp) trigger expression of the tryptophanase tnaCAB operon in E. coli. Activation depends on tryptophan-dependent ribosomal stalling during translation of the upstream TnaC peptide. Here, we present a cryoelectron microscopy (cryo-EM) reconstruction at 3.8 Å resolution of a ribosome stalled by the TnaC peptide. Unexpectedly, we observe two L-Trp molecules in the ribosomal exit tunnel coordinated within composite hydrophobic pockets formed… CONTINUE READING
Related Discussions
This paper has been referenced on Twitter 2 times. VIEW TWEETS


Publications citing this paper.
Showing 1-10 of 22 extracted citations

How Widespread is Metabolite Sensing by Ribosome-Arresting Nascent Peptides?

Journal of molecular biology • 2016
View 14 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…