Molecular basis for the glyphosate‐insensitivity of the reaction of 5‐enolpyruvylshikimate 3‐phosphate synthase with shikimate

@article{Priestman2005MolecularBF,
  title={Molecular basis for the glyphosate‐insensitivity of the reaction of 5‐enolpyruvylshikimate 3‐phosphate synthase with shikimate},
  author={Melanie A. Priestman and M. Healy and T. Funke and A. Becker and E. Sch{\"o}nbrunn},
  journal={FEBS Letters},
  year={2005},
  volume={579}
}
The shikimate pathway enzyme 5‐enolpyruvyl shikimate‐3‐phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad‐spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate‐3‐phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1… Expand
18 Citations
Characterization of a class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate from Sinorhizobium fredii
  • L. Wang, R. Peng, +5 authors Q. Yao
  • Biology, Medicine
  • World journal of microbiology & biotechnology
  • 2014
  • 5
Crystal structure of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase from the ESKAPE pathogen Acinetobacter baumannii.
  • 9
  • PDF
Functional characterization of aroA from Rhizobium leguminosarum with significant glyphosate tolerance in transgenic Arabidopsis.
  • J. Han, Y. Tian, +4 authors Q. Yao
  • Medicine, Biology
  • Journal of microbiology and biotechnology
  • 2014
  • 3
Over-expression of the Gr5aroA gene from glyphosate-contaminated soil confers high tolerance to glyphosate in tobacco
  • 8
The Effects of Herbicides Targeting Aromatic and Branched Chain Amino Acid Biosynthesis Support the Presence of Functional Pathways in Broomrape
  • 1
  • Highly Influenced
...
1
2
...

References

SHOWING 1-10 OF 28 REFERENCES
Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail.
  • 401
  • PDF
The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase.
  • 867
Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site.
  • 145
Substrate synergism and the steady-state kinetic reaction mechanism for EPSP synthase from Escherichia coli.
  • 46
The shikimate pathway and its branches in apicomplexan parasites.
  • 126
  • PDF
...
1
2
3
...