Molecular basis for the deficiency in humans of gulonolactone oxidase, a key enzyme for ascorbic acid biosynthesis.

@article{Nishikimi1991MolecularBF,
  title={Molecular basis for the deficiency in humans of gulonolactone oxidase, a key enzyme for ascorbic acid biosynthesis.},
  author={Morimitsu Nishikimi and Ken Yagi},
  journal={The American journal of clinical nutrition},
  year={1991},
  volume={54 6 Suppl},
  pages={
          1203S-1208S
        }
}
The inability of humans to synthesize L-ascorbic acid is known to be due to a lack of L-gulono-gamma-lactone oxidase, an enzyme that is required for the biosynthesis of this vitamin. Isolation of a cDNA for rat L-gulono-gamma-lactone oxidase allowed us to study the basic defect underlying this deficiency at the gene level and led to isolation of a human genomic clone related to L-gulono-gamma-lactone oxidase as well as three overlapping clones covering the entire coding region of the rat L… CONTINUE READING

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